Investigation towards the biohalogenation of the chlorinated natural products from Fischerella ambigua (Näg.) Gomont:
Gespeichert in:
| Beteilige Person: | |
|---|---|
| Format: | Hochschulschrift/Dissertation Buch |
| Sprache: | Englisch |
| Veröffentlicht: |
München
Verl. Dr. Hut
2008
|
| Ausgabe: | 1. Aufl. |
| Schriftenreihe: | Pharmazeutische Biologie
|
| Schlagwörter: | |
| Links: | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016999345&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Umfang: | XIII, 181 S. Ill., graph. Darst. |
| ISBN: | 9783899638172 |
Internformat
MARC
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| 245 | 1 | 0 | |a Investigation towards the biohalogenation of the chlorinated natural products from Fischerella ambigua (Näg.) Gomont |c vorgelegt von Wagner, Claudia |
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Datensatz im Suchindex
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|---|---|
| adam_text | STERILIZATION PROCEDURES TABLE OF CONTENT III TABLE OF CONTENT I
VORVEROFFENTLICHUNGEN / ALREADY PUBLISHED RESULTS 1 II ACKNOWLEDGEMENTS
LIT III TABLE OF CONTENT VII IV ABBREVIATIONS XI 1 INTRODUCTION 1 1.1
HALOGENATED NATURAL PRODUCTS 2 1.1.1 NOVEL THERAPEUTICALLY PROMISING
MARINE-DERIVED HALOGENATED NATURAL PRODUCTS 3 1.1.2 CYANOBACTERIA AS
ORIGIN OF HALOGENATED NATURAL PRODUCTS 4 1.1.3 MICROORGANISMS AS ORIGIN
OF MARINE DERIVED METABOLITES 11 1.2 HALOGENATING ENZYMES 14 1.2.1
HALOPEROXIDASES 14 1.2.2 FADHZ-DEPENDENT HALOGENASES 21 1.2.3 FURTHER
HALOGENATING ENZYMES 40 1.3 FISCHERELLA AMBIGUA 45 2 INTENT OF THE
PRESENTED STUDY 47 3 MATERIALS AND METHODS 49 49 49 51 52 52 53 55 56 59
60 3.1 3.1.1 3.1.2 3.1.3 3.1.4 3.1.5 3.1.6 3.1.7 3.2 3.3 MATERIALS
CHEMICALS AND OTHER MATERIALS ENZYMES MOLECULAR WEIGHT MARKERS MOLECULAR
BIOLOGICAL KIDS OLIGONUCLEOTIDES CULTURE MEDIA BUFFERS AND SOLUTIONS
SOFTWARE AND DATABASES BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/991285352 DIGITALISIERT DURCH VIII TABLE OF CONTENT 3.4
CULTIVATION OF CYANOBACTERIAL ORGANISM 60 3.4.1 ORIGIN OF CYANOBACTERIAL
FISCHERELLA AMBIGUA 60 3.4.2 MAINTENANCE AS STOCK CULTURE 60 3.4.3
SMALL-SCALE CULTIVATION FOR DNA ISOLATION 60 3.4.4 LARGE-SCALE
CULTIVATION FOR ANALYTICAL PURPOSES AND PROTEIN PURIFICATION 60 3.5
ANALYTICAL TECHNIQUES 61 3.5.1 THIN LAYER CHROMATOGRAPHY (TLC) 61 3.5.2
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) 61 3.5.3 PROTON NUCLEAR
MAGNETIC RESONANCE SPECTROSCOPY ( 1 H-NMR) 61 3.5.4 MASS SPECTROSCOPY
(MS) 62 3.5.5 UV7VIS SPECTROSCOPY 63 3.6 CHEMICAL ANALYSIS OF
FISCHERELLA AMBIGUA NATURAL PRODUCT FORMATION 63 3.6.1 CRUDE EXTRACT
PREPARATION 63 3.6.2 CRUDE EXTRACT CHARACTERISATION 63 3.7 PROTEIN
PURIFICATION 64 3.7.1 PREPARATION OF THE CRUDE EXTRACT 64 3.7.2 FAST
PROTEIN LIQUID CHROMATOGRAPHY (FPLC) 64 3.7.3 POLYACRYLAMIDE GEL
ELECTROPHORESIS (SDS-PAGE) 65 3.7.4 DETERMINATION OF PROTEIN
CONCENTRATIONS 66 3.7.5 MONOCHLORODIMEDONE ASSAY 67 3.8 MOLECULAR
BIOLOGICAL METHODS 69 3.8.1 ISOLATION OF CHROMOSOMAL DNA 69 3.8.2
AGAROSE GEL ELECTROPHORESIS 72 3.8.3 DNA EXTRACTION FROM AGAROSE GEL 72
3.8.4 POLYMERASE CHAIN REACTION (PCFT) 72 3.8.5 RESTRICTION DIGESTION 74
3.8. TABLE OF CONTENT 4.2 4.2.1 4.2.2 4.2.3 4.2.4 4.3 4.3.1 4.3.2 4.3.3
4.3.4 5 5.1 5.2 5.2.1 5.2.2 5.3 5.3.1 5.3.2 5.3.3 5.3.4 5.4 6 7 8
IDENTIFICATION OF A PROTEIN ACTIVE IN THE HALOPEROXIDASE ASSAY PROTEIN
PURIFICATION CHARACTERIZATION OF THE PRODUCTS FORMED WITHIN THE ASSAY BY
CYTOCHROME C PROTEINS TEST OF AMBIGOL-PRECURSOR-LIKE STRUCTURES WITHIN
THE ASSAY REACTION IN ABSENCE OF HALIDE IONS HALOGENASE SCREENING
ISOLATION OF CHROMOSOMAL DNA SOUTHERN BLOT HYBRIDIZATION PCR SCREENING
FURTHER SPECIFICATION OF THE PUTATIVE F. AMBIGUA HALOGENASE SEQUENCES
DISCUSSION BIOACTIVE NATURAL PRODUCTS FROM FISCHERELLA AMBIGUA
HALOPEROXIDASE ACTIVITY GUIDED PROTEIN PURIFICATION CRITICAL VIEW ON THE
MONOCHLORODIMEDONE-ASSAY CONCLUSION OF THE HPO ACTIVITY GUIDED PROTEIN
PURIFICATION HALOGENASE SCREENING COMPARISON OF METHODS FOR ISOLATION OF
CHROMOSOMAL DNA FROM F. AMBIGUA FADH2-DEPENDING HALOGENASES WITHIN F.
AMBIGUA CONSIDERATION
|
| any_adam_object | 1 |
| author | Wagner, Claudia 1974- |
| author_GND | (DE-588)136757936 |
| author_facet | Wagner, Claudia 1974- |
| author_role | aut |
| author_sort | Wagner, Claudia 1974- |
| author_variant | c w cw |
| building | Verbundindex |
| bvnumber | BV035192752 |
| ctrlnum | (OCoLC)645010346 (DE-599)DNB991285352 |
| dewey-full | 615.19 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 615 - Pharmacology and therapeutics |
| dewey-raw | 615.19 |
| dewey-search | 615.19 |
| dewey-sort | 3615.19 |
| dewey-tens | 610 - Medicine and health |
| discipline | Medizin |
| edition | 1. Aufl. |
| format | Thesis Book |
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| genre_facet | Hochschulschrift |
| id | DE-604.BV035192752 |
| illustrated | Illustrated |
| indexdate | 2024-12-20T13:25:19Z |
| institution | BVB |
| isbn | 9783899638172 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-016999345 |
| oclc_num | 645010346 |
| open_access_boolean | |
| owner | DE-12 DE-29T DE-19 DE-BY-UBM |
| owner_facet | DE-12 DE-29T DE-19 DE-BY-UBM |
| physical | XIII, 181 S. Ill., graph. Darst. |
| publishDate | 2008 |
| publishDateSearch | 2008 |
| publishDateSort | 2008 |
| publisher | Verl. Dr. Hut |
| record_format | marc |
| series2 | Pharmazeutische Biologie |
| spellingShingle | Wagner, Claudia 1974- Investigation towards the biohalogenation of the chlorinated natural products from Fischerella ambigua (Näg.) Gomont Halogenierung (DE-588)4292867-9 gnd Fischerella ambigua (DE-588)4420290-8 gnd Sekundärmetabolit (DE-588)4300564-0 gnd |
| subject_GND | (DE-588)4292867-9 (DE-588)4420290-8 (DE-588)4300564-0 (DE-588)4113937-9 |
| title | Investigation towards the biohalogenation of the chlorinated natural products from Fischerella ambigua (Näg.) Gomont |
| title_auth | Investigation towards the biohalogenation of the chlorinated natural products from Fischerella ambigua (Näg.) Gomont |
| title_exact_search | Investigation towards the biohalogenation of the chlorinated natural products from Fischerella ambigua (Näg.) Gomont |
| title_full | Investigation towards the biohalogenation of the chlorinated natural products from Fischerella ambigua (Näg.) Gomont vorgelegt von Wagner, Claudia |
| title_fullStr | Investigation towards the biohalogenation of the chlorinated natural products from Fischerella ambigua (Näg.) Gomont vorgelegt von Wagner, Claudia |
| title_full_unstemmed | Investigation towards the biohalogenation of the chlorinated natural products from Fischerella ambigua (Näg.) Gomont vorgelegt von Wagner, Claudia |
| title_short | Investigation towards the biohalogenation of the chlorinated natural products from Fischerella ambigua (Näg.) Gomont |
| title_sort | investigation towards the biohalogenation of the chlorinated natural products from fischerella ambigua nag gomont |
| topic | Halogenierung (DE-588)4292867-9 gnd Fischerella ambigua (DE-588)4420290-8 gnd Sekundärmetabolit (DE-588)4300564-0 gnd |
| topic_facet | Halogenierung Fischerella ambigua Sekundärmetabolit Hochschulschrift |
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