Gene Cloning: the mechanics of DNA manipulation
Gespeichert in:
| Beteilige Person: | |
|---|---|
| Format: | Buch |
| Sprache: | Nichtbestimmte Sprache |
| Veröffentlicht: |
London <<[u.a.]>>
Chapman and Hall
1984
|
| Ausgabe: | Rev. ed. [d. Ausg.]: Genetic engineering, cloning DNA. 2. ed. |
| Schriftenreihe: | Outline studies in biology
|
| Schlagwörter: | |
| Links: | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018516143&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Umfang: | VIII, 222 S. Ill., graph. Darst. |
| ISBN: | 0412254301 0412266008 |
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Datensatz im Suchindex
| _version_ | 1819288514033876992 |
|---|---|
| adam_text | IMAGE 1
CONTENTS
PREFACE VII
1 THE PRINCIPLES OF CLONING DNA 1
1.1 THE DEBATE ON THE SAFETY OF WORK WITHIN RECOMBI- NANT DNA 2
1.2 GENERAL PRINCIPLES OF CLONING 4
1.3 STRATEGIES FOR GENE CLONING 8
1.4 FUNCTIONAL STUDIES WITH CLONED GENES 18
REFERENCES 19
2 RECOMBINATION AND MUTAGENESIS OF DNA IN VITRO 2 1 2.1 THE ENZYMOLOGY
OF IN VITRO DNA RECOMBINATION 21 2.2 THE SYNTHESIS OF DNA FOR CLONING 30
2.3 IN VITRO MUTAGENESIS 37
REFERENCES 45
3 BACTERIOPHAGE VECTORS 48
3.1 BACTERIOPHAGE A 48
3.2 SINGLE-STRANDED DNA PHAGES 72
REFERENCES 81
4 BACTERIAL PLASM ID VECTORS 85
4.1 VECTORS FOR E. COLI 86
4.2 VECTORS FOR OTHER GRAM NEGATIVE ORGANISMS 97
4.3 VECTORS FOR GRAM POSITIVE ORGANISMS 102
REFERENCES 106
IMAGE 2
VI CONTENTS
5 EXPRESSION OF CLONED DN AS IN E. COLI PLASMIDS 110
5.1 PROMOTERS 110
5.2 FUSION PROTEINS - A MEANS TO INCREASE THE STABILITY OF FOREIGN
PEPTIDES IN E. COLI 115
5.3 SECRETION OF PROTEINS FROM E. COLI 120
5.4 FACTORS AFFECTING THE TRANSLATION OF HYBRID GENES 121 5.5
POST-TRANSLATIONAL MODIFICATION 125
REFERENCES 126
6 THE PHYSICAL CHARACTERIZATION OF CLONED DNA SEGMENTS AND THEIR
COUNTERPARTS WITHIN CHROMOSOMES 128
6.1 PHYSICAL CHARACTERIZATION OF CLONED DNA 129
6.2 DETECTING SPECIFIC NUCLEIC ACID SEQUENCES WITHIN HETEROGENEOUS
POPULATIONS OF MOLECULES - GEL TRANS- FER HYBRIDIZATION 141
6.3 MAPPING CLONED DNA TO CHROMOSOMAL LOCI 146
6.4 GENETIC DISEASES 150
REFERENCES 155
7 GENE CLONING IN FUNGI AND PLANTS 158
7.1 SACCHAROMYCES CEREVISIAE 158
7.2 FILAMENTOUS FUNGI 167
7.3 CLONING DNA IN PLANT CELLS - TI PLASMID 168
REFERENCES 175
8 EXPRESSION OF CLONED GENES IN ANIMAL CELLS 179
8.1 DIRECT TRANSFORMATION OF MAMMALIAN CELLS 179
8.2 SV40 VECTORS 182
8.3 ADENOVIRUS VECTORS 193
8.4 BOVINE PAPILLOMA VIRUS VECTORS 197
8.5 RETROVIRAL VECTORS 198
8.6 VACCINIA VIRUS VECTORS 203
8.7 MAXIMIZING EXPRESSION 206
8.8 WHOLE ANIMAL TRANSFORMATION 208
REFERENCES 213
INDEX 219
|
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| bvnumber | BV024542313 |
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| discipline | Biologie |
| edition | Rev. ed. [d. Ausg.]: Genetic engineering, cloning DNA. 2. ed. |
| format | Book |
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| id | DE-604.BV024542313 |
| illustrated | Illustrated |
| indexdate | 2024-12-20T13:57:34Z |
| institution | BVB |
| isbn | 0412254301 0412266008 |
| language | Undetermined |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-018516143 |
| oclc_num | 251793590 |
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| owner_facet | DE-83 |
| physical | VIII, 222 S. Ill., graph. Darst. |
| psigel | TUB-nvm |
| publishDate | 1984 |
| publishDateSearch | 1984 |
| publishDateSort | 1984 |
| publisher | Chapman and Hall |
| record_format | marc |
| series2 | Outline studies in biology |
| spellingShingle | Glover, David M. Gene Cloning the mechanics of DNA manipulation Genklonierung (DE-588)4123275-6 gnd Klonierung (DE-588)4192480-0 gnd Rekombinante DNS (DE-588)4225578-8 gnd |
| subject_GND | (DE-588)4123275-6 (DE-588)4192480-0 (DE-588)4225578-8 |
| title | Gene Cloning the mechanics of DNA manipulation |
| title_auth | Gene Cloning the mechanics of DNA manipulation |
| title_exact_search | Gene Cloning the mechanics of DNA manipulation |
| title_full | Gene Cloning the mechanics of DNA manipulation David M. Glover |
| title_fullStr | Gene Cloning the mechanics of DNA manipulation David M. Glover |
| title_full_unstemmed | Gene Cloning the mechanics of DNA manipulation David M. Glover |
| title_short | Gene Cloning |
| title_sort | gene cloning the mechanics of dna manipulation |
| title_sub | the mechanics of DNA manipulation |
| topic | Genklonierung (DE-588)4123275-6 gnd Klonierung (DE-588)4192480-0 gnd Rekombinante DNS (DE-588)4225578-8 gnd |
| topic_facet | Genklonierung Klonierung Rekombinante DNS |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018516143&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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