Pharmacological modulation of the Th1/Th2 balance: a new concept for anti-asthmatic therapy
Gespeichert in:
| Beteilige Person: | |
|---|---|
| Format: | Hochschulschrift/Dissertation Buch |
| Sprache: | Englisch Deutsch |
| Veröffentlicht: |
2003
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| Schlagwörter: | |
| Links: | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=010600398&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| Beschreibung: | Erlangen-Nürnberg, Univ., Diss., 2003. - Enth. dt. Zs.fassung |
| Umfang: | III, 100 S. Ill., graph. Darst. |
Internformat
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Datensatz im Suchindex
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| adam_text | PHARMACOLOGICAL MODULATION OF THE TH1/TH2 BALANCE: A NEW CONCEPT FOR
ANTI-ASTHMATIC THERAPY DEN NATURWISSENSCHAFTLICHEN FAKULTATEN DER
FRIEDRICH-ALEXANDER UNIVERSITAT ERLANGEN-NIIRNBERG ZUR ERLANGUNG DES
DOKTORGRADES VORGELEGT VON MEIXIA ZHANG AUS SHANDONG, P.R. CHINA
CONTENTS 1 INTRODUCTION 1 1.1 PATHOPHYSIOLOGY OF ASTHMA 1 1.2 THL TH2
PARADIGM 2 1.3 T CELL ACTIVATION 3 1.4 ANTI-INFLAMMATORY THERAPY OF
ASTHMA 6 1.5 MECHANISM OF APOPTOSIS 7 1.6 AIM OF THE STUDY 9 2 MATERIALS
AND METHODS 11 2.1 MATERIALS 11 2.1.1 REAGENTS 11 2.1.2 PRIMERS AND
PROBES 14 2.2 METHODS 14 2.2.1 PREPARATION OF PBMC 14 2.2.2 PREPARATION
OF CD4+T CELL 14 2.2.3 PREPARATION OF MONOCYTES 15 2.2.4 CELL
PROLIFERATION .7. 15 2.2.5 CELL TREATMENT AND CULTURE 15 2.2.6 CULTURE
OF CELL LINES 15 2.2.7 RT-PCR 16 2.2.8 ELISA 16 2.2.9 MEASUREMENT OF
HUMAN CD4+T CELL ANERGY 16 2.2.10 CHANGES OF PHOSPHATIDYLSERINE
EXTERNALIZATION AND CELL MEMBRANE IMPAIRMENT * ANNEXIN-V AND PI ASSAY 17
2.2.11 CHANGES OF MITOCHONDRIAL MEMBRANE POTENTIAL * JC-1 ASSAY 17
2.2.12 CASPACE FITC-VAD-FMK IN SITU MARKER LABELING 18 CONTENTS 2.2.13
CASPATAG CASPASE-6 ACTIVITY ASSAY 18 2.2.14 WESTERN BLOTTING ANALYSIS 19
3 RESULTS 21 3.1 HUMAN IL-2, IL-4, IL-5 AND IFN-Y REGULATION BY
DIFFERENT IMMUNOMODULATING DRUGS 21 3.1.1 TIME COURSE OF IL-2, IL-4,
IL-5 AND IFN-Y INDUCTION IN HUMAN PBMC 21 3.1.2 EFFECTS OF INHIBITORS OF
THE CALCIUM-*NF-AT PATHWAY 21 3.1.3 EFFECTS OF NF-KB INHIBITORS 26 3.1.4
EFFECTS OF MAPK INHIBITORS 29 3.1.5 IC50 VALUES OF DIFFERENT DRUGS FOR
IL-2, IL-4,11-5 AND IFN-Y MRNA IN HUMAN PBMC 34 3.2 THE EFFECTS OF
DIFFERENT IMMUNOMODULATING DRUGS ON HUMAN IL-13 34 3.2.1 INDUCTION OF
IL-13 IN HUMAN PBMCS 35 3.2.2 TIME COURSE OF IL-13 INDUCTION 36 3.2.3
EFFECTS OF INHIBITORS OF THE CALCIUM-»NF-AT PATHWAY 37 3.2.4 EFFECTS OF
NF-KB INHIBITORS 40 3.2.5 EFFECTS OF MAPK INHIBITORS 43 3.2.6 COMPARISON
OF IC 50 OF PBMC WITH CD4+ T CELLS 46 3.3 THE EFFECT OF D-43787 ON HUMAN
T CELL AND PROINFLAMMATORY CYTOKINES .47 3.3.1 DOSE RESPONSE EFFECT ON
CELL PROLIFERATION 47 3.3.2 D-43787 DOES NOT INDUCE ANERGY IN HUMAN CD4+
T CELLS 49 3.3.3 D-43787 INHIBITS TH2 CYTOKINES 50 3.3.4 DOSE RESPONSE
EFFECT ON THL/TH2 CYTOKINES 54 3.3.5 DOSE RESPONSE EFFECT ON
PROINFLAMMATORY CYTOKINES 56 3.4 APOPTOTIC EFFECT OF D-43787 ON HUMAN
CD4+ T CELLS 58 CONTENTS III 3.4.1 D-43787 INDUCES APOPTOSIS IN HUMAN
CD4+ T CELLS 58 3.4.2 INDUCTION OF APOPTOSIS DEPENDS ON A SPECIFIC
COMPOUND STRUCTURE AND A NEW BINDING SITE AT CYCLOPHILIN 60 3.4.3
D-43787 INDUCES DEPOLARIZATION OF MITOCHONDRIA IN CD4+ T CELLS.. 63
3.4.4 D-43787 ACTIVATES CASPASE-LIKE PROTEASES 64 3.4.5 CASPASE-6
MEDIATES INDUCTION OF APOPTOSIS BY D-43787 65 3.4.6 CLEAVAGE OF THE
CASPASE-6 SUBSTRATE LAMIN A/C 68 4 DISCUSSION 71 4.1 THE EFFECTS OF
DIFFERENT IMMUNOSUPPRESSIVE DRUGS ON HUMAN IL-2, IL-4, IL-5 AND IFN-Y
REGULATION 72 4.2 THE EFFECTS OF DIFFERENT IMMUNOSUPPRESSIVE DRUGS ON
HUMAN IL-13 REGULATION 75 4.3 THE EFFECT OF D-43787 ON HUMAN T CELL
CYTOKINES AND PROINFLAMMATORY CYTOKINES 77 4.4 APOPTOTIC EFFECTS OF
D-43787 ON HUMAN CD4+ T CELLS 79 5 SUMMARY 83 6 ZUSAMMENFASSUNG 85 7
REFERENCE 89 8 ACKNOWLEDGEMENT 101 9 CURRICULUM VITAE 102
|
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| illustrated | Illustrated |
| indexdate | 2024-12-20T11:21:18Z |
| institution | BVB |
| language | English German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-010600398 |
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| physical | III, 100 S. Ill., graph. Darst. |
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| spellingShingle | Zhang, Meixia 1964- Pharmacological modulation of the Th1/Th2 balance a new concept for anti-asthmatic therapy Helferzelle (DE-588)4258130-8 gnd Therapie (DE-588)4059798-2 gnd Allergisches Asthma (DE-588)4275602-9 gnd |
| subject_GND | (DE-588)4258130-8 (DE-588)4059798-2 (DE-588)4275602-9 (DE-588)4113937-9 |
| title | Pharmacological modulation of the Th1/Th2 balance a new concept for anti-asthmatic therapy |
| title_auth | Pharmacological modulation of the Th1/Th2 balance a new concept for anti-asthmatic therapy |
| title_exact_search | Pharmacological modulation of the Th1/Th2 balance a new concept for anti-asthmatic therapy |
| title_full | Pharmacological modulation of the Th1/Th2 balance a new concept for anti-asthmatic therapy von Meixia Zhang |
| title_fullStr | Pharmacological modulation of the Th1/Th2 balance a new concept for anti-asthmatic therapy von Meixia Zhang |
| title_full_unstemmed | Pharmacological modulation of the Th1/Th2 balance a new concept for anti-asthmatic therapy von Meixia Zhang |
| title_short | Pharmacological modulation of the Th1/Th2 balance |
| title_sort | pharmacological modulation of the th1 th2 balance a new concept for anti asthmatic therapy |
| title_sub | a new concept for anti-asthmatic therapy |
| topic | Helferzelle (DE-588)4258130-8 gnd Therapie (DE-588)4059798-2 gnd Allergisches Asthma (DE-588)4275602-9 gnd |
| topic_facet | Helferzelle Therapie Allergisches Asthma Hochschulschrift |
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